Crystal structures of Ophiostoma piceae sterol esterase: Structural insights into activation mechanism and product release
نویسندگان
چکیده
منابع مشابه
Potential of Ophiostoma piceae sterol esterase for biotechnologically relevant hydrolysis reactions
The ascomycete Ophiostoma piceae produces a sterol esterase (OPE) with high affinity toward p-nitrophenol, glycerol, and sterol esters. Recently, this enzyme has been heterologously expressed in the methylotrophic yeast Pichia pastoris under the AOX1 methanol-inducible promoter (PAOX1) using sorbitol as co-susbtrate, and the hydrolytic activity of the recombinant protein (OPE*) turned out to be...
متن کاملRecombinant sterol esterase from Ophiostoma piceae: an improved biocatalyst expressed in Pichia pastoris
BACKGROUND The ascomycete Ophiostoma piceae produces a sterol esterase (OPE) with high affinity towards p-nitrophenol, glycerol and sterol esters. Its hydrolytic activity on natural mixtures of triglycerides and sterol esters has been proposed for pitch biocontrol in paper industry since these compounds produce important economic losses during paper pulp manufacture. RESULTS Recently, this en...
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ژورنال
عنوان ژورنال: Journal of Structural Biology
سال: 2014
ISSN: 1047-8477
DOI: 10.1016/j.jsb.2014.07.007